Stable transformation of a Mamestra brassicae (lepidoptera) cell line with the lepidopteran-derived transposon piggyBac
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چکیده
منابع مشابه
The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly.
The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector-helper system in the Mediterranean fruit fly Ceratitis capitata. A piggyBac vector marked with the medfly white gene was tested with a normally regulated piggyBac transposase helper at two different concentrati...
متن کاملGermline transformation of Drosophila melanogaster with the piggyBac transposon vector.
Germline transformation of Drosophila melanogaster was attempted with the piggyBac gene-transfer system from the cabbage looper moth, Trichoplusia ni. Using a self-regulated transposase helper and a white marked vector, a transformation frequency of 1-3% per fertile G0 was obtained, similar to that previously achieved in the medfly. Use of an hsp70-regulated helper increased this frequency more...
متن کاملCharacterization of a UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase activity in a Mamestra brassicae cell line.
The binding of Bandeiraea simplicifolia lectin-I isolectin B4 on the endogenous glycoproteins of different insect cell lines led us to characterize for the first time a UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase in a Mamestra brassicae cell line (Mb). The study of the acceptor specificity indicated that the Mb alpha-galactosyltransferase prefers Galbeta1-3-R as acceptor, and among...
متن کاملAnnual Migration of Cabbage Moth, Mamestra brassicae L. (Lepidoptera: Noctuidae), over the Sea in Northern China
The cabbage moth, Mamestra brassicae L. (Lepidoptera: Noctuidae), is a serious pest of vegetable crops throughout the world. In order to determine whether or not M. brassicae is a migrant, and if yes, what is the pattern of M. brassicae seasonal migration, a long-term study on M. brassicae from April to October in 2003-2014 was carried out by means of a searchlight trap on a small island locate...
متن کاملPlant volatiles activating specific olfactory receptor neurons of the cabbage moth Mamestra brassicae L. (Lepidoptera, Noctuidae).
Herbivore insects are suitable model organisms for studying how plant odor information is encoded in olfactory receptor neurons (RNs). By the use of gas chromatography linked to electrophysiological recordings from single RNs, screening for sensitivity to naturally produced plant odorants is possible in order to determine the molecular receptive ranges of the neurons. Using this method, we have...
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ژورنال
عنوان ژورنال: Insect Biochemistry and Molecular Biology
سال: 2003
ISSN: 0965-1748
DOI: 10.1016/s0965-1748(02)00189-3